One Kit, Two Applications- Accurate Results in Minutes
Flow CAST® is intended for the determination of basophil activation in whole blood samples by flow cytometry. Our unique marker combination (CCR3 and CD63) combines the simple gating properties for basophil detection by CCR3 with the solid application of the proven activation marker CD63 in one test. Thanks to its easy protocol and smart marker combination the first results can be obtained in under an hour which provides perfect conditions for laboratories.
- Versatile and Robust: Solid applications for allergy testing and for screening in vitro and ex vivo studies
- Best Standardized Reagents for Basophil Activation: Optimized stimulation buffer, specific activators, and accurate quantification
- World-Class Support: Gold standard publications, excellent scientific/technical customer support
- QUICK: 30 minutes to quantified basophil activation inhibition
Kinase Inhibition Application
Simplifying Drug Development
BÜHLMANN’s Flow CAST Assay® for Kinase Inhibition offers a quick and precise means to evaluate inhibitors of immune cell signaling pathway, using inhibition of basophil degranulation as a surrogate read out cell based system by flow cytometry.
Allergy in a vial, Surrogate for OFC (Oral Food Challenge)
BÜHLMANN’s Flow CAST ®Assay for Allergy provides an efficient method for cellular allergy stimulation testing using a broad range of quality controlled allergens and mixes which are standardized and validated for use in this assay and the CAST® ELISA.
Learn more about kinase inhibition and check out the Flow CAST® in testing and optimizing kinase inhibitors in drug development.
Flow CAST® is backed by numerous publications in the food allergy field. Check out this basophil activation test (BAT) relevant literature list.
Basophil Activation Testing- Standardized
Flow CAST® Procedure Steps (Overview)
View Product Flyers
Learn more about Flow CAST® in our technical product flyer and take a closer look at pre-analytics as well as assay procedure, specifications, performance and more.
See our full allergen list, product specifications, and ordering details in English and French below.